Author(s): Nampoothiri SV, Prathapan A, Cherian OL, Raghu KG, Venugopalan VV, , Nampoothiri SV, Prathapan A, Cherian OL, Raghu KG, Venugopalan VV, , Nampoothiri SV, Prathapan A, Cherian OL, Raghu KG, Venugopalan VV, , Nampoothiri SV, Prathapan A, Cherian OL, Raghu KG, Venugopalan VV,
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Abstract The present study evaluated the free radical scavenging capacity and antioxidant potential of different solvent extracts (Hexane (HE), ethyl acetate (EA), methanol (ME), 70\% methanol (MW) and Water (WA)) of Terminalia bellerica (TB) and Emblica officinalis (EB) fruits. Methanol extract (ME) of TB and EB fruits exhibited maximum scavenging activity against DPPH, superoxide, hydroxyl and nitric oxide radicals. Cell based antioxidant activity was assayed by flow cytometry using DCFH-DA as probe. Methanol extracts were also screened for their antidiabetic activity via inhibition of α-amylase, α-glucosidase and antiglycation assays. Results showed that ME of TB and EB can act as potent α-amylase and α-glucosidase inhibitor. Significant antiglycation activity also confirms the therapeutic potential of these extracts against diabetes. Both the extracts significantly inhibited the oxidation of LDL under in vitro conditions. Liquid chromatography-mass spectroscopy (LC-MS) analysis revealed that methanol extract of TB and EB contains ellagic acid and ascorbic acid as the major compound respectively. Copyright © 2010 Elsevier Ltd. All rights reserved.
This article was published in Food Chem Toxicol
and referenced in Journal of Nutritional Disorders & Therapy