alexa In vitro biosynthesis of isoprene from mevalonate utilizing a rat liver cytosolic fraction.
Genetics & Molecular Biology

Genetics & Molecular Biology

Journal of Molecular Biomarkers & Diagnosis

Author(s): Deneris ES, Stein RA, Mead JF

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Abstract The in vitro biosynthesis of isoprene from DL-mevalonate in the cytosolic fraction of rat liver is described. Evidence is provided suggesting a non-enzymatic formation of isoprene from isopentenyl pyrophosphate and/or dimethylallyl pyrophosphate. Furthermore, the data establish an alternate fate of the mevalonate carbon skeleton providing the first evidence that breath isoprene is linked to cholesterol biosynthesis.
This article was published in Biochem Biophys Res Commun and referenced in Journal of Molecular Biomarkers & Diagnosis

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