alexa In Vivo and In Vitro Cross Neutralization Studies of Local Rabies Virus Isolates with ERA Based Cell Culture Anti-Rabies Vaccine Produced In Ethiopia
Infectious Diseases

Infectious Diseases

Journal of Infectious Diseases & Preventive Medicine

Author(s): Abebe M Aga, Yalem Mekonnen, Birhanu Hurisa, Kelbessa Urga

Abstract Share this page

Rabies is a worldwide problem, and the case is most severe in developing countries where cell culture anti-rabiesvaccines are unaffordable or the available nervous tissue-derived vaccines are of questionable immunogenicity andmay cause neurological complications. The aim of this research was to study cross protection of local rabies virusisolates with Evenyl Roktincki Abelseth (ERA) based cell culture anti-rabies vaccine produced in Ethiopia and todevelop challenge virus from local isolates. The viruses were isolated from rabid dogs’ brains and human saliva, andadapted on Swiss albino mice and cell lines. Cross protection with ERA based vaccine was studied by in vivo and invitro methods. For in vivo method, a group of mice were immunized on day zero and seven with 0.5 ml (1:5 dilutions)of ERA based cell culture anti-rabies vaccine produced locally. On day fourteenth, mice were challenged withworking dilution of each local isolates and one group with challenge virus standard (CVS-11), and observed forfurther 14 days. High protection was recorded in CVS-11 challenged mice and low protection in all local isolates(p=0.045); specifically protection to HOS challenged mice was very low. In vitro test was done by fluorescentantibody virus neutralization (FAVN) test on BHK-21 cell lines. Sera from dog immunized with locally producedvaccine and OIE serum were incubated with local virus isolates and CVS-11 for 48 hours in the presence of celllines. Maximum antibody titer (2.74 IU/ml) was obtained with CVS-11 challenge virus and minimum antibody titer(1.55 IU/ml) was obtained with cow origin (CO) virus isolate. All locally isolated rabies virus show low antibody titerwhen compared to CVS-11 and PV-12 (p=0.000). From the results, it can be concluded that local isolates havesome genetic variation from fixed virus strain which can affect efficacy of the candidate vaccine and potency valueshould be set in-terms of local isolate using as challenge virus. Generally, the exact genetic relationship should bestudied by molecular techniques and locally isolated virus should be used as challenge virus for vaccine qualitycontrol.

This article was published in Vaccines & Vaccination and referenced in Journal of Infectious Diseases & Preventive Medicine

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

agrifoodaquavet@omicsonline.com

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

clinical_biochem@omicsonline.com

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

business@omicsonline.com

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

chemicaleng_chemistry@omicsonline.com

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

environmentalsci@omicsonline.com

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

engineering@omicsonline.com

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

generalsci_healthcare@omicsonline.com

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

genetics_molbio@omicsonline.com

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

immuno_microbio@omicsonline.com

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

omics@omicsonline.com

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

materialsci@omicsonline.com

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

mathematics_physics@omicsonline.com

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

medical@omicsonline.com

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

neuro_psychology@omicsonline.com

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

pharma@omicsonline.com

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

social_politicalsci@omicsonline.com

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version