alexa In vivo gene delivery. Efficient transfection of T lymphocytes in adult mice.
Genetics & Molecular Biology

Genetics & Molecular Biology

Journal of Genetic Syndromes & Gene Therapy

Author(s): Philip R, Liggitt D, Philip M, Dazin P, Debs R

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Abstract We have demonstrated that a single intraperitoneal injection of cationic liposomes complexed to a chloramphenicol acetyltransferase (CAT) gene expression plasmid can transfect the majority of splenic Thy 1.2+ T lymphocytes, as well as significant numbers of bone marrow-derived hematopoietic cells, in adult mice. CAT activity was detected in the spleen for at least 2 weeks, and there was no evidence of treatment-related toxicity. Some degree of tissue-specific transgene expression was achieved by varying the cationic lipid used.
This article was published in J Biol Chem and referenced in Journal of Genetic Syndromes & Gene Therapy

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