alexa Inactivation of formyltransferase (wbkC) gene generates a Brucella abortus rough strain that is attenuated in macrophages and in mice.
General Science

General Science

Journal of Bioterrorism & Biodefense

Author(s): Lacerda TL, Cardoso PG, Augusto de Almeida L, Camargo IL, Afonso DA,

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Abstract Rough mutants of Brucella abortus were generated by disruption of wbkC gene which encodes the formyltransferase enzyme involved in LPS biosynthesis. In bone marrow-derived macrophages the B. abortusDeltawbkC mutants were attenuated, could not reach a replicative niche and induced higher levels of IL-12 and TNF-alpha when compared to parental smooth strains. Additionally, mutants exhibited attenuation in vivo in C57BL/6 and interferon regulatory factor-1 knockout mice. DeltawbkC mutant strains induced lower protective immunity in C56BL/6 than smooth vaccine S19 but similar to rough vaccine RB51. Finally, we demonstrated that Brucella wbkC is critical for LPS biosynthesis and full bacterial virulence. Copyright 2010 Elsevier Ltd. All rights reserved. This article was published in Vaccine and referenced in Journal of Bioterrorism & Biodefense

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