Author(s): Shi Y, Xia J, Yang TH
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Abstract OBJECTIVE: To study the inhibitory effect of arsenic trioxide (As2O3) on the pulmonary metastasis of melanoma B16 cells. METHODS: Mice melanoma cells B16 were injected into the vein of the eye socket of C57BL/6J mice. The lung tissue weight and the B16 melanoma lung metastasis nodules were examined after intraperitoneal injection of As2O3. The microvessel density in the pulmonary metastatic tumor nodules was observed using HE staining and immunohistochemistry analysis for VIII-R Ag. The cell adhesion rate was detected using CellTiter 96 Aqueous One reagent. RESULTS: As2O3 could significantly inhibit the pulmonary metastasis of B16 melanoma. The lung weight, the pulmonary metastasis nodules, and microvessels per visual field of the experimental group and the control group were 0.139+/-0.013 g and 0.353+/-0.070 g, 20.42+/-1.78 and 61.42+/-3.09, 3.25+/-0.75 and 7.50+/-1.45, respectively (all P<0.01). As2O3 showed significant effect on the cell adhesion rate, showing statistical difference between the two groups (P<0.01). CONCLUSIONS: As2O3 had significant antitumor metastasis effect. It might be correlated with inhibiting angiogenesis and enhancing the cell adhesion.
This article was published in Zhongguo Zhong Xi Yi Jie He Za Zhi
and referenced in Journal of Integrative Oncology