Author(s): Smithies O, Gregg RG, Boggs SS, Koralewski MA, Kucherlapati RS
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Abstract A 'rescuable' plasmid containing globin gene sequences allowing recombination with homologous chromosomal sequences has enabled us to produce, score and clone mammalian cells with the plasmid integrated into the human beta-globin locus. The planned modification was achieved in about one per thousand transformed cells whether or not the target gene was expressed.
This article was published in Nature
and referenced in Advancements in Genetic Engineering