alexa In-situ immuno-gold nanoparticle network ELISA biosensors for pathogen detection.
Materials Science

Materials Science

Journal of Nanomedicine & Nanotechnology

Author(s): Cho IH, Irudayaraj J

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Abstract Food poisoning microorganisms that contaminate food products and compromise food safety and security have been considered a major health threat and a serious concern for food producers and processors. Developing sensor technologies that are rapid for sensitive and selective detection and quantification of pathogens is a high priority for scientists in academia, state and federal research institutes, and industries. In this work we propose an in-situ immuno-AuNP network-based ELISA biosensor integrated with a sample concentration step based on immuno-magnetic separation to detect pathogenic microorganisms with high sensitivity. The sensor system was optimized by the specific formation of immuno-AuNP network onto the antigenic site present at the outer membrane surface of bacteria and the analytical concept was validated by a microtiter immunoassay. The in-situ network biosensor was able to detect pathogens at extremely low numbers: 3 cells/mL of Escherichia coli O157:H7 and Salmonella typhimurium in buffer and 3 CFU/mL of E. coli O157:H7 and 15CFU/mL of S. typhimurium in real sample conditions within 2h of inoculation. The ability to monitor target bacteria with improved analytical sensitivity compared to the current techniques presents a unique opportunity for routine monitoring to improve the safety of foods. Copyright © 2013 Elsevier B.V. All rights reserved. This article was published in Int J Food Microbiol and referenced in Journal of Nanomedicine & Nanotechnology

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