alexa Interaction of natural and synthetic polynucleotides with liposomes in the presence of divalent cations
Microbiology

Microbiology

Journal of Chemical Biology & Therapeutics

Author(s): Kuvichkin VV, Sukhomudrenko AG

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The formation of complexes of polynucleotides (DNA, poly A.poly U) with liposomes from egg lecithins, L-alpha-phosphatidylcholine, dimirystoyl and other lipids in the presence of divalent cations was studied by differential scanning microcalorimetry circular dichroism and turbidimetry. It was shown that the secondary structure of polynucleotides (double or triple helix) was necessary for the formation of these complexes. This structure was partially destroyed during formation of complexes. It was shown, that three main types of lipids, i.e. phosphatidylcholine, phosphatidylethanolamine and sphingomyelin participate in interactions between liposomes, polynucleotides and Mg2+.

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This article was published in Biofizika and referenced in Journal of Chemical Biology & Therapeutics

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