alexa Interfacing microsampling droplets and mass spectrometry by paper spray ionization for online chemical monitoring of cell culture.
Chemical Engineering

Chemical Engineering

Journal of Chromatography & Separation Techniques

Author(s): Liu W, Wang N, Lin X, Ma Y, Lin JM

Abstract Share this page

Abstract In this work, the establishment of a microdialysis-paper spray ionization-mass spectrometry (MS) system was described. A homemade microdialysis module was employed for sampling, and microdroplets were generated at the outlet of the capillary conducting the dialysate. Online MS analysis of each microdroplet was immediately accomplished, interfacing by paper spray ionization. Analytical performance of the method was investigated and improved through the introduction of thinner capillary tubes and the optimization of spray solvent and paper substrate. For microdroplets with concentrated salt at 50 nL, the limit of detection at 0.8 ppm (or 40 pg absolute) and a highest resolution at about 1.5 s were achieved. The integrated system was applied into the online monitoring of glucose concentration in cell culture mediums. A satisfactory linearity of the calibration curve between the relative MS intensity and the glucose concentration was observed. Furthermore, as a model, hormone regulation of the glucose concentration was investigated. This work demonstrated the potential application of the label-free, online "MS sensor" into studies on cellular metabolism. This article was published in Anal Chem and referenced in Journal of Chromatography & Separation Techniques

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version