alexa Intracellular localisation, geno- and cytotoxic response of polyN-isopropylacrylamide (PNIPAM) nanoparticles to human keratinocyte (HaCaT) and colon cells (SW 480).
Materials Science

Materials Science

Journal of Nanomedicine & Nanotechnology

Author(s): Naha PC, Bhattacharya K, Tenuta T, Dawson KA, Lynch I,

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Abstract PNIPAM nanoparticles, with and without a covalently linked fluorescent label, were prepared by a free radical polymerisation technique. The cyto- and genotoxicity of PNIPAM nanoparticles were analysed in two representative mammalian cell lines, SW480, a colon, and HaCaT, a dermal cell line. Physical characterisation in terms of particle size and zeta potential of the PNIPAM nanoparticles was carried out both in aqueous solution and in the appropriate cell culture media. Uptake and co-localisation of fluorescently labelled PNIPAM nanoparticles was monitored in both cell lines using confocal laser scanning microscope. Genotoxicity analysis using the Comet assay was performed in both cell lines to evaluate any DNA damage. It was observed that the PNIPAM nanoparticles were internalized and localised in lysosomes within 24h. No significant cytotoxic response (p
  • DOI: 10.1016/j.toxlet.2010.06.011
  • This article was published in Toxicol Lett and referenced in Journal of Nanomedicine & Nanotechnology

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