alexa Investigating crystal-growth mechanisms with and without LB template: protein transfer from LB to crystal.
Microbiology

Microbiology

Journal of Microbial & Biochemical Technology

Author(s): Pechkova E, Fiordoro S, Fontani D, Nicolini C

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Abstract Protein nanocrystallography has recently been introduced as a unique nanotechnology-based approach to forming stable protein crystals and to characterize them down to atomic resolution. In particular, a protein nanostuctured template appears to be capable of stimulating nucleation and crystal growth of so far unsolved proteins. In the present communication, aimed at investigating the lysozyme crystal-growth mechanisms with and without nanotemplate, the lysozymes appear to transfer directly from the nanostructured film into the drop to trigger the formation of the crystal, therefore highlighting the physical interpretation of the mechanism for nanotemplate-induced protein crystallization. This article was published in Acta Crystallogr D Biol Crystallogr and referenced in Journal of Microbial & Biochemical Technology

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