alexa Investigation of the mechanism of protein adsorption on ordered mesoporous silica using flow microcalorimetry.
Chemistry

Chemistry

Industrial Chemistry

Author(s): Katiyar A, Thiel SW, Guliants VV, Pinto NG

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Abstract The adsorption of bovine serum albumin (BSA) and lysozyme (LYS) on siliceous SBA-15 with 24 nm pores was studied using flow microcalorimetry; this is the first attempt to understand the thermodynamics of protein adsorption on SBA-15 using flow microcalorimetry. The adsorption mechanism is a strong function of protein structure. Exothermic events were observed when protein-surface interactions were attractive. Entropy-driven endothermic events were also observed in some cases, resulting from lateral protein-protein interactions and conformational changes in the adsorbed protein. The magnitudes of the enthalpies of adsorption for primary protein-surface interactions decrease with increased surface coverage, indicating the possibility of increased repulsion between adsorbed protein molecules. Secondary exothermic events were observed for BSA adsorption, presumably due to secondary adsorption made possible by conformational changes in the soft BSA protein. These secondary adsorption events were not observed for lysozyme, which is structurally robust. The results of this study emphasize the influence of solution conditions and protein structure on conformational changes of the adsorbed protein and the value of calorimetry in understanding protein-surface interactions. 2010 Elsevier B.V. All rights reserved. This article was published in J Chromatogr A and referenced in Industrial Chemistry

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