alexa Investigation of the poultry idiotypic network using Pasteurella multocida.
Immunology

Immunology

Journal of Vaccines & Vaccination

Author(s): Zhang H, Ainsworth AJ

Abstract Share this page

Abstract The idiotypic network in leghorn laying hens was investigated by inoculating hens with a 35.5 kD outer membrane protein of Pasteurella multocida (Pm35.5), idiotype (Id), or Pm35.5 anti-Id. Egg yolks were analyzed for the presence of Id, anti-Id, or anti-anti-Id. Anti-Pm35.5 antibodies (Id) were not titered to an end-point but were present in high concentrations. The presence of anti-Id antibody in yolk was demonstrated by the inhibition of Pm35.5 binding to Id by anti-Id using a flow microsphere inhibition immunoassay. Inhibition of Pm35.5 binding to Id caused by different anti-Id preparations ranges from 51.5 to 56.1\%. Not all of the anti-Id bound to a paratope-associated Id, since 8.3-12.8\% of the fluoresceinated anti-Id bound to Id-coated beads in the presence of excess Pm35.5. We confirmed that a portion of the anti-Id antibodies was an internal image of the Pm35.5 Id and could mimic antigen by demonstrating that anti-Id inoculated in naive hens caused the synthesis of anti-anti-Id antibodies that bound to Pm35.5 in enzyme linked immunosorbent assay. Monoclonal anti-Id antibodies were also capable of inhibiting Pm35.5 binding to Pm35.5 Id coated microspheres and inducing anti-anti-Id antibodies in BALB/cJ mice that reacted with the original antigen, Pm35.5. Our investigation has also shown that idiotypic antibodies to P. multocida were transferred from chicken to egg.
This article was published in Vet Immunol Immunopathol and referenced in Journal of Vaccines & Vaccination

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords