Author(s): Zhang H, Ainsworth AJ
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Abstract The idiotypic network in leghorn laying hens was investigated by inoculating hens with a 35.5 kD outer membrane protein of Pasteurella multocida (Pm35.5), idiotype (Id), or Pm35.5 anti-Id. Egg yolks were analyzed for the presence of Id, anti-Id, or anti-anti-Id. Anti-Pm35.5 antibodies (Id) were not titered to an end-point but were present in high concentrations. The presence of anti-Id antibody in yolk was demonstrated by the inhibition of Pm35.5 binding to Id by anti-Id using a flow microsphere inhibition immunoassay. Inhibition of Pm35.5 binding to Id caused by different anti-Id preparations ranges from 51.5 to 56.1\%. Not all of the anti-Id bound to a paratope-associated Id, since 8.3-12.8\% of the fluoresceinated anti-Id bound to Id-coated beads in the presence of excess Pm35.5. We confirmed that a portion of the anti-Id antibodies was an internal image of the Pm35.5 Id and could mimic antigen by demonstrating that anti-Id inoculated in naive hens caused the synthesis of anti-anti-Id antibodies that bound to Pm35.5 in enzyme linked immunosorbent assay. Monoclonal anti-Id antibodies were also capable of inhibiting Pm35.5 binding to Pm35.5 Id coated microspheres and inducing anti-anti-Id antibodies in BALB/cJ mice that reacted with the original antigen, Pm35.5. Our investigation has also shown that idiotypic antibodies to P. multocida were transferred from chicken to egg.
This article was published in Vet Immunol Immunopathol
and referenced in Journal of Vaccines & Vaccination