alexa Ionic liquid-assisted electrospray ionization of polysaccharides.
Chemical Engineering

Chemical Engineering

Mass Spectrometry & Purification Techniques

Author(s): Chang YL, Lee YC, Yang WB, Chen CH

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Abstract In this work, we give the report of significant detection sensitivity improvement of electrospray ionization (ESI) mass spectra of polysaccharides by adding various ionic liquid compounds into samples. Mass spectra obtained were greatly simplified and appeared to be similar to spectra from matrix-assisted laser desorption/ionization due to the narrow charge number distribution. Mass spectra of polysaccharides with the attachment of either anion or cation of ionic liquid compounds were observed. No protonated or deprotonated polysaccharide ions were detected when ionic liquid compounds were added into samples. Little alkali-attached polysaccharide ions were observed. Ionic liquid-assisted ESI (ILA-ESI) mass spectrometry has significantly improved the detection sensitivity of large neutral polysaccharide compounds. Copyright © 2011 John Wiley & Sons, Ltd. This article was published in J Mass Spectrom and referenced in Mass Spectrometry & Purification Techniques

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