alexa Is an intracellular renin-angiotensin system involved in control of cell communication in heart?

Journal of Autacoids and Hormones

Author(s): De Mello WC

Abstract Share this page

Abstract The possible influence of an intracellular renin-angiotensin system (RAS) on control of cell communication in heart muscle was investigated in cell pairs isolated from adult rats. Junctional conductance (gj) was measured with two separated voltage-clamp circuits. Intracellular dialysis of angiotensin I (AI 10(-8) M) caused a decrease in gj of 76\% (SE +/- 3.4) (p < 0.05) in 7 min. The effect of AI appears to be due mainly to its conversion to AII because enalaprilat (10(-9) M) dialysed into the cell caused an appreciable reduction in the effect of AI. AII (10(-8) M) alone caused a decrease in gj of 60\% (SE +/- 3.8) (p < 0.05) in 45 s. The effect of AII on gj was suppressed by previous inhibition of protein kinase C (PKC), but enalaprilat could not alter the effect of the peptide. The results indicate that synthesis of AII inside cardiac myocytes plays an important role in modulation of gj and consequently on propagation of the electrical impulse in heart. The effect of AII on gj was blocked by DuP-753 (10(-9) M) administered intracellularly, whereas (Sar1Val5AlA8) AII also caused a slight decrease (1.97 +/- 0.07\%) in gj. These findings indicate that an intracellular receptor is involved in the effect of the peptide on gj.
This article was published in J Cardiovasc Pharmacol and referenced in Journal of Autacoids and Hormones

Relevant Expert PPTs

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version