alexa Isolation and characterization of membranes from the cultivated mushroom.


Journal of Experimental Food Chemistry

Author(s): Holtz RB, Stewart PS, Patton S

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Abstract The membranes of sporophore cap tissue from the cultivated mushroom, Agaricus bisporus (Lange) Sing., were isolated using discontinuous sucrose gradient ultracentrifugation of a tissue homogenate. A membrane-rich fraction was concentrated at the 1.16/1.18 g/cc interface and a mitochondria-rich fraction at the 1.18/1.20 g/cc interface. The membrane fraction was judged to be greater than 90\% membrane vesicles by electron microscopy. The protein to lipid ratio of the membrane fraction was 1.1; the molar ratio of sterol to phospholipid was 0.77. The specific radioactivity of a Mg-activated ATPase was 2.5 times greater in the membrane fraction than in the homogenate. No 5'-nucleotidase or Na-K-Mg-activated ATPase activity was observed.
This article was published in Plant Physiol and referenced in Journal of Experimental Food Chemistry

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