alexa Isolation and characterization of polycyclic aromatic hydrocarbon-degrading bacteria associated with the rhizosphere of salt marsh plants.
Environmental Sciences

Environmental Sciences

Journal of Bioremediation & Biodegradation

Author(s): Daane LL, Harjono I, Zylstra GJ, Hggblom MM

Abstract Share this page

Abstract Polycyclic aromatic hydrocarbon (PAH)-degrading bacteria were isolated from contaminated estuarine sediment and salt marsh rhizosphere by enrichment using either naphthalene, phenanthrene, or biphenyl as the sole source of carbon and energy. Pasteurization of samples prior to enrichment resulted in isolation of gram-positive, spore-forming bacteria. The isolates were characterized using a variety of phenotypic, morphologic, and molecular properties. Identification of the isolates based on their fatty acid profiles and partial 16S rRNA gene sequences assigned them to three main bacterial groups: gram-negative pseudomonads; gram-positive, non-spore-forming nocardioforms; and the gram-positive, spore-forming group, Paenibacillus. Genomic digest patterns of all isolates were used to determine unique isolates, and representatives from each bacterial group were chosen for further investigation. Southern hybridization was performed using genes for PAH degradation from Pseudomonas putida NCIB 9816-4, Comamonas testosteroni GZ42, Sphingomonas yanoikuyae B1, and Mycobacterium sp. strain PY01. None of the isolates from the three groups showed homology to the B1 genes, only two nocardioform isolates showed homology to the PY01 genes, and only members of the pseudomonad group showed homology to the NCIB 9816-4 or GZ42 probes. The Paenibacillus isolates showed no homology to any of the tested gene probes, indicating the possibility of novel genes for PAH degradation. Pure culture substrate utilization experiments using several selected isolates from each of the three groups showed that the phenanthrene-enriched isolates are able to utilize a greater number of PAHs than are the naphthalene-enriched isolates. Inoculating two of the gram-positive isolates to a marine sediment slurry spiked with a mixture of PAHs (naphthalene, fluorene, phenanthrene, and pyrene) and biphenyl resulted in rapid transformation of pyrene, in addition to the two- and three-ringed PAHs and biphenyl. This study indicates that the rhizosphere of salt marsh plants contains a diverse population of PAH-degrading bacteria, and the use of plant-associated microorganisms has the potential for bioremediation of contaminated sediments.
This article was published in Appl Environ Microbiol and referenced in Journal of Bioremediation & Biodegradation

Relevant Expert PPTs

Relevant Speaker PPTs

  • Dassanayake TDMSB
    Comparison of BMI, body fat percentage and lean body weight of national level teenage swimmers and non-swimmers in Sri Lanka
    PPT Version | PDF Version
  • Rasha Mosa
    Effects of growth hormone secretagogue receptor agonist and antagonist in non-obese Type 2 Diabetes MKR mice
    PPT Version | PDF Version
  • Trent Jackman
    Exploratory comparison of credentialed clinical instructors’ and non-credentialed clinical instructors’ report of frequency and importance of professional behaviors in clinical education
    PPT Version | PDF Version
  • Saraswathi K
    Diagnosis of different stages of non-proliferative diabetic retinopathy
    PPT Version | PDF Version
  • Guldeniz Karadeniz Cakmak
    The importance of intraoperative ultrasound guidance to achieve negative margins for palpable and nonpalpable breast cancer
    PPT Version | PDF Version
  • Elena Bonanno
    Microcalcification as an active phenomenon mediated by epithelial cells with mesenchymal characteristics
    PPT Version | PDF Version
  • Antoine N. Gergess
    Antoine N. Gergess-University-of-Balamand-Lebanon-Cold-Bending-Steel-Beams-a-State-of-The-Art-Engineering-Solution-that-Meets-Industry-Challenges
    PPT Version | PDF Version
  • Gabriela Briceno
    Selection of an actinobacteria consortium for enhancing diazinon degradation
    PPT Version | PDF Version
  • Osama O. Ibrahim
    Chemicals structure, properties, regulations and applications of non-nutritive-high intensity sweeteners
    PDF Version
  • Wendy peters
    A non-replicating Ad5 vaccine for treatment of HSV-2
    PPT Version | PDF Version
  • Yosef Yarden
    Classically, the 3’untranslated region (3’UTR) is that region in eukaryotic protein-coding genes from the translation termination codon to the polyA signal. It is transcribed as an integral part of the mRNA encoded by the gene. However, there exists another kind of RNA, which consists of the 3’UTR alone, without all other elements in mRNA such as 5’UTR and coding region. The importance of independent 3’UTR RNA (referred as I3’UTR) was prompted by results of artificially introducing such RNA species into malignant mammalian cells. Since 1991, we found that the middle part of the 3’UTR of the human nuclear factor for interleukin-6 (NF-IL6) or C/EBP gene exerted tumor suppression effect in vivo. Our subsequent studies showed that transfection of C/EBP 3’UTR led to down-regulation of several genes favorable for malignancy and to up-regulation of some genes favorable for phenotypic reversion. Also, it was shown that the sequences near the termini of the C/EBP 3’UTR were important for its tumor suppression activity. Then, the C/EBP 3’UTR was found to directly inhibit the phosphorylation activity of protein kinase CPKC in SMMC-7721, a hepatocarcinoma cell line. Recently, an AU-rich region in the C/EBP 3’UTR was found also to be responsible for its tumor suppression. Recently we have also found evidence that the independent C/EBP 3’UTR RNA is actually exists in human tissues, such as fetal liver and heart, pregnant uterus, senescent fibroblasts etc. Through 1990’s to 2000’s, world scientists found several 3’UTR RNAs that functioned as artificial independent RNAs in cancer cells and resulted in tumor suppression. Interestingly, majority of genes for these RNAs have promoter-like structures in their 3’UTR regions, although the existence of their transcribed products as independent 3’UTR RNAs is still to be confirmed. Our studies indicate that the independent 3’UTR RNA is a novel non-coding RNA species whose function should be the regulation not of the expression of their original mRNA, but of some essential life activities of the cell as a whole.
    PPT Version | PDF Version
  • Ding-Gan Liu
    Independent 3’untranslated region RNA: A novel non-coding regulator RNA
    PPT Version | PDF Version
  • Sergey Alekseenko
    Sergey-Alekseenko-Institute-of-Thermophysics-Russia-Nonlinear-waves-and-transfer-processes-in-liquid-film-flow
    PPT Version | PDF Version
  • Ewa Maj
    Antiangiogenic treatment in non-small cell lung cancer (NSCLC)
    PPT Version | PDF Version
  • Zakaria Hmama
    A non-genetic approach to improve the tuberculosis vaccine BCG
    PPT Version | PDF Version

Recommended Conferences

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords