Author(s): Malghani S, Chatterjee N, Yu HX, Luo Z
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Abstract An enrichment culture technique was used to isolate bacterial strains responsible for the biodegradation of profenofos in a soil from Hubei province of central China. Two pure bacterial cultures, named W and Y, were isolated and subsequently characterized by sequencing of 16S rRNA genes and biochemical tests. Isolate W showed 96\% similarity to the 16S rRNA gene of a Pseudomonas putida unlike Y which showed 99\% similarity to the 16S rRNA gene of Burkholderia gladioli. Both strains grew well at pH 5.5-7.2 with a broad temperature profile ranging from 28° to 36 °C. Bioremediation of profenofos-contaminated soil was examined using soil treated with 200 ug g(-1); profenofos resulted in a higher degradation rate than control soils without inoculation. In a mineral salt medium (FTW) reduction in profenofos concentration was 90\% within 96 hours of incubation. A literature survey revealed that no data is available regarding the role of Burkholderia gladioli on pesticide biodegradation as well as on profenofos.
This article was published in Braz J Microbiol
and referenced in Journal of Bioremediation & Biodegradation