Author(s): Hoffman RM, Wood TM
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Abstract Clearing of agar plates containing ball-milled, delignified straw has been used for screening mutants of Penicillium funiculosum IMI 87160 iii. The effects of glycerol and a number of sugars on the clearing were investigated for selecting derepressed mutants. The beta-glucosidase synthesis by one such mutant, C22c, in shake flasks containing straw was not repressed by 5\% glycerol, whereas activities on filter paper, CM-cellulose, and p-nitrophenyl-beta-xylosidase were only partially derepressed; xylanase was extensively derepressed. The evidence for separate control of the enzymes involved in the solubilization of straw is discussed.
This article was published in Biotechnol Bioeng
and referenced in Journal of Bioprocessing & Biotechniques