alexa Isolation and properties of Ca2+-transporting glycoprotein and peptide from beef heart mitochondria.
Biochemistry

Biochemistry

Biochemistry & Analytical Biochemistry

Author(s): Mironova GD, Sirota TV, Pronevich LA, Trofimenko NV, Mironov GP,

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Abstract The 40,000-dalton glycoprotein and 2000-dalton peptide inducing selective Ca2+-transport through bilayer lipid membranes were isolated from beef heart homogenate and mitochondria. Micromolar concentrations of these substances were found to increase the conductivity of membranes by 3-4 orders. Trans-membrane Ca2+ gradient induces an electric potential difference whose magnitude is close to the theoretical for ideal Ca2+ selectivity. The inhibitor of mitochondrial Ca2+ transport, ruthenium red, abolishes both the glycoprotein- and peptide-induced Ca2+ transport in bilayer lipid membranes. Thiol groups essential for Ca2+ transport activity were revealed in the glycoprotein and peptide. Addition of these substances to rat liver mitochondria induces Ca2+-dependent inhibition of the state 3 respiration that can be released by uncouplers (oligomycin-like effect).
This article was published in J Bioenerg Biomembr and referenced in Biochemistry & Analytical Biochemistry

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