alexa Isolation of high quality RNA from Streptomyces.


Journal of Addiction Research & Therapy

Author(s): Van Dessel W, Van Mellaert L, Geukens N, Lammertyn E, Ann J

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Abstract Several molecular techniques require high quality RNA, completely free of DNA. Standard methods to isolate total RNA from Streptomyces spp. are based on the application of a 'Modified Kirby Mix' [Practical Streptomyces Genetics. The John Innes Foundation, Norwich, pp. 613]. Here we present an alternative procedure using Triton X-100 and EDTA for the isolation of total RNA from Streptomyces. Copyright 2004 Elsevier B.V. This article was published in J Microbiol Methods and referenced in Journal of Addiction Research & Therapy

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