alexa Kinetic analysis on formation of poly(3-hydroxybutyrate) from acetic acid by Ralstonia eutropha under chemically defined conditions.
Environmental Sciences

Environmental Sciences

Journal of Bioremediation & Biodegradation

Author(s): Wang J, Yu J

Abstract Share this page

Abstract Batch cultures of Ralstonia eutropha in chemically defined media with acetic acid (HAc) as the sole carbon source were conducted to investigate acetate utilization, formation of poly(3-hydroxybutyrate) (PHB) and growth of active biomass (ABM) under different carbon to nitrogen (C/N) weight ratios. The specific acetate utilization rate based on ABM approached 0.16 g/g ABM h(-1), which was not affected very much by the extracellular HAc concentration from 1 to 5 g/l, but was affected by the C/N weight ratio. A low C/N ratio or high nitrogen supply sped up the specific acetate utilization rate to produce more ABM and less PHB. A high HAc concentration (>6 g/l), however, depressed acetate utilization as well as the ABM growth and PHB formation. A high cell mass concentration enhanced the tolerance of R. eutropha to the toxicity of HAc at pH 7 to 8.5. The viscosity-average molecular size of PHB generally increased first and then declined in batch cultures. Larger PHB molecules and less PHB per ABM were produced at a low C/N ratio with enough nutrient nitrogen than those under a high C/N ratio with less nutrient nitrogen available. This article was published in J Ind Microbiol Biotechnol and referenced in Journal of Bioremediation & Biodegradation

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

  • 6th World Congress on Biofuels and Bioenergy
    Sep 5-6, 2017 London, UK
  • 6th World Congress on Biopolymers
    September 7-9, 2017 Paris, France
  • 7th International Conference and Exhibition on Biopolymers and Bioplastics
    October 19-21, 2017 San Francisco, USA
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version