alexa Kinetic study of hepatic triglyceride lipase from rat liver soluble fraction.
Chemical Engineering

Chemical Engineering

Journal of Analytical & Bioanalytical Techniques

Author(s): RuizLarrea MF, GaldizValdovinos B, RodrguezFernndez C

Abstract Share this page

Abstract An assay is described for the determination of lipase activity, using as substrate an emulsion of radioactive triolein with cholic acid as emulsifier. Lipase activity is given by determining radioactive triolein disappearance after incubation with the artificial emulsion. The catalytic activity of this enzyme has been studied with respect to time, protein concentration and substrate concentration. Under the experimental conditions, Michaelis constant value was 14.54 mmol/l and maximal hydrolysis rate 0.31 mumol h-1 mg-1.
This article was published in Enzyme and referenced in Journal of Analytical & Bioanalytical Techniques

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version