alexa Label-free and time-resolved measurements of cell volume changes by surface plasmon resonance (SPR) spectroscopy.
General Science

General Science

Journal of Biotechnology & Biomaterials

Author(s): Robelek R, Wegener J

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Abstract Cell volume and its regulation is one of the key players for cellular integrity and a strong indicator for several cell pathologies. But time-resolved volume measurements of adherently grown mammalian cells using established methods, such as extracellular impedance analysis or light microscopy, are complex and time-consuming. In this study, we demonstrate that surface plasmon resonance spectroscopy (SPR) is a powerful transducer device capable of reporting volume changes of cells that are directly grown on the SPR sensor surface. The approach is label-free, non-invasive and provides an outstanding time resolution. In proof-of-principle studies we recorded the volume change of confluent MDCK II cells induced by hypo- or hypertonic stimulation in a time-resolved manner. Comparison of the SPR-based experiments reported here with more recent studies using different approaches suggests a direct correlation between SPR signal shift and cell volume changes. Copyright 2009 Elsevier B.V. All rights reserved. This article was published in Biosens Bioelectron and referenced in Journal of Biotechnology & Biomaterials

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