alexa Landscape phage probes for Salmonella typhimurium.
Engineering

Engineering

Biosensors Journal

Author(s): Sorokulova IB, Olsen EV, Chen IH, Fiebor B, Barbaree JM, , Sorokulova IB, Olsen EV, Chen IH, Fiebor B, Barbaree JM,

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Abstract We selected from landscape phage library probes that bind preferentially Salmonella typhimurium cells compared with other Enterobacteriaceae. The specificity of the phage probes for S. typhimurium was analyzed by the phage-capture test, the enzyme-linked immunosorbent assay (ELISA), and the precipitation test. Interaction of representative probes with S. typhimurium was characterized by fluorescence-activated cell sorting (FACS), and fluorescent, optical and electron microscopy. The results show that the landscape phage library is a rich source of specific and robust probes for S. typhimurium suitable for long-term use in continuous monitoring devices and biosorbents. This article was published in J Microbiol Methods and referenced in Biosensors Journal

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