alexa Large scale extraction of poly(3-hydroxybutyrate) from Ralstonia eutropha H16 using sodium hypochlorite.
Environmental Sciences

Environmental Sciences

Journal of Petroleum & Environmental Biotechnology

Author(s): Heinrich D, Madkour MH, AlGhamdi MA, Shabbaj II, Steinbchel A

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Abstract Isolation of polyhydroxyalkanoates (PHAs) from bacterial cell matter is a critical step in order to achieve a profitable production of the polymer. Therefore, an extraction method must lead to a high recovery of a pure product at low costs. This study presents a simplified method for large scale poly(3-hydroxybutyrate), poly(3HB), extraction using sodium hypochlorite. Poly(3HB) was extracted from cells of Ralstonia eutropha H16 at almost 96\% purity. At different extraction volumes, a maximum recovery rate of 91.32\% was obtained. At the largest extraction volume of 50 L, poly(3HB) with an average purity of 93.32\% ┬▒ 4.62\% was extracted with a maximum recovery of 87.03\% of the initial poly(3HB) content. This process is easy to handle and requires less efforts than previously described processes.
This article was published in AMB Express and referenced in Journal of Petroleum & Environmental Biotechnology

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