alexa Light-dependent generation of reactive oxygen species in cell culture media.
Molecular Biology

Molecular Biology

Journal of Cytology & Histology

Author(s): Grzelak A, Rychlik B, Bartosz G

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Abstract Cell culture media (RPMI 1640, Dulbecco's Minimal Essential Medium and yeast extract-peptone-glucose medium) were found to oxidize dichlorodihydrofluorescein diacetate and dihydrorhodamine 123, and to generate spin adduct of 5,5'-dimethyl-1-pyrroline N-oxide, which indicates formation of reactive oxygen species (ROS). The production of ROS was light dependent. The main component of the media responsible for the generation of ROS was riboflavin, but tryptophan, tyrosine, pyridoxine, and folic acid enhanced the effect of riboflavin. These observations point to exposure of cells to ROS under in vitro culture conditions.
This article was published in Free Radic Biol Med and referenced in Journal of Cytology & Histology

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