Author(s): Hanson JR, Macalady JL, Harris D, Scow KM
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Abstract Phospholipid fatty acid (PLFA) analysis of a soil microbial community was coupled with (13)C isotope tracer analysis to measure the community's response to addition of 35 microg of [(13)C]toluene ml of soil solution(-1). After 119 h of incubation with toluene, 96\% of the incorporated (13)C was detected in only 16 of the total 59 PLFAs (27\%) extracted from the soil. Of the total (13)C-enriched PLFAs, 85\% were identical to the PLFAs contained in a toluene-metabolizing bacterium isolated from the same soil. In contrast, the majority of the soil PLFAs (91\%) became labeled when the same soil was incubated with [(13)C]glucose. Our study showed that coupling (13)C tracer analysis with PLFA analysis is an effective technique for distinguishing a specific microbial population involved in metabolism of a labeled substrate in complex environments such as soil.
This article was published in Appl Environ Microbiol
and referenced in Journal of Aquaculture Research & Development