alexa Lipase-mediated transformation of vegetable oils into biodiesel using propan-2-ol as acyl acceptor.
Biochemistry

Biochemistry

Enzyme Engineering

Author(s): Modi MK, Reddy JR, Rao BV, Prasad RB

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Abstract Propan-2-ol was used as an acyl acceptor for immobilized lipase-catalyzed preparation of biodiesel. The optimum conditions for transesterification of crude jatropha (Jatropha curcas), karanj (Pongamia pinnata) and sunflower (Helianthus annuus) oils were 10\% Novozym-435 (immobilized Candida antarctica lipase B) based on oil weight, alcohol to oil molar ratio of 4:1 at 50 degrees C for 8 h. The maximum conversions achieved using propan-2-ol were 92.8, 91.7 and 93.4\% from crude jatropha, karanj and sunflower oils, respectively. Reusability of the lipase was maintained over 12 repeated cycles with propan-2-ol while it reached to zero by 7(th) cycle when methanol was used as an acyl acceptor, under standard reaction conditions. This article was published in Biotechnol Lett and referenced in Enzyme Engineering

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