Author(s): Fang X, Liu Y, Kong J, Jiang X, Fang X, Liu Y, Kong J, Jiang X, Fang X, Liu Y, Kong J, Jiang X, Fang X, Liu Y, Kong J, Jiang X
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Abstract This work shows that loop-mediated isothermal amplification (LAMP) of nucleic acid can be integrated in an eight-channel microfluidic chip for readout either by the naked eye (as a result of the insoluble byproduct pyrophosphate generating during LAMP amplification) or via absorbance measured by an optic sensor; we call this system microLAMP (microLAMP). It is capable of analyzing target nucleic acids quantitatively with high sensitivity and specificity. The assay is straightforward in manipulation. It requires a sample volume of 0.4 microL and is complete within 1 h. The sensitivity of the assay is comparable to standard methods, where 10 fg of DNA sample could be detected under isothermal conditions (63 degrees C). A real time quantitative microLAMP assay using absorbance detection is possible by integration of optical fibers within the chip.
This article was published in Anal Chem
and referenced in Research & Reviews: Journal of Botanical Sciences