alexa Low level of DNA repair in human chromosome 1 heterochromatin.
Genetics & Molecular Biology

Genetics & Molecular Biology

Journal of Molecular Biomarkers & Diagnosis

Author(s): Surralls J, Darroudi F, Natarajan AT

Abstract Share this page

Abstract Band 1q12 is a breakage prone region commonly involved in chromosome 1 rearrangements found in human cancer. We have investigated whether a lack of DNA repair can account for the observed 1q12 fragility. Ethyl methanesulfonate (EMS)-induced repair sites originating in G0/G1 phase of the cell cycle were converted to chromosome breaks by blocking the re-synthesis step of excision repair with cytosine arabinoside. Thus, unfilled repair gaps reached S-phase and were detected as chromosome breaks after cell division. Chromosome breakage in the overall genome was monitored by scoring of micronuclei. Breaks in 1q12 were detected in interphase lymphocytes by in situ hybridization with tandem probes targeting the chromosome 1 centromere (D1Z5) and the adjacent heterochromatic band 1q12(pUC1.77). Mitomycin-C (0.12 and 0.24 micrograms/ml) and X-rays (1 and 2 Gy) were used as positive controls to check the suitability of the tandem labelling approach in detecting 1q12 clastogenicity. Our data indicated that, although the methodology was suitable to detect 1q12 breakage and a high level of overall genome DNA repair occurred, there was a low level of EMS-induced DNA repair sites in 1q12 converted to chromosome breaks. This finding was consistent throughout G1 phase, suggesting that there is a relatively low level of DNA excision repair in human chromosome 1 heterochromatin.
This article was published in Genes Chromosomes Cancer and referenced in Journal of Molecular Biomarkers & Diagnosis

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords