alexa Map-based cloning of a protein kinase gene conferring disease resistance in tomato.
Environmental Sciences

Environmental Sciences

Journal of Biodiversity, Bioprospecting and Development

Author(s): Martin GB, Brommonschenkel SH, Chunwongse J, Frary A, Ganal MW,

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Abstract The Pto gene in tomato confers resistance to races of Pseudomonas syringae pv. tomato that carry the avirulence gene avrPto. A yeast artificial chromosome clone that spans the Pto region was identified and used to probe a leaf complementary DNA (cDNA) library. A cDNA clone was isolated that represents a gene family, at least six members of which genetically cosegregate with Pto. When susceptible tomato plants were transformed with a cDNA from this family, they were resistant to the pathogen. Analysis of the amino acid sequence revealed similarity to serine-threonine protein kinases, suggesting a role for Pto in a signal transduction pathway.
This article was published in Science and referenced in Journal of Biodiversity, Bioprospecting and Development

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