Author(s): Jemmerson R, Paterson Y, Jemmerson R, Paterson Y
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Abstract A monoclonal antibody bound to a protein antigen decreases the rate of proteolytic cleavage of the antigen, having the greatest effect on those regions involved in antibody contact. Thus, an epitope can be identified by the ability of the antibody to protect one region of the antigen more than others from proteolysis. By means of this approach, two distinct epitopes, both conformationally well-ordered, were characterized on horse cytochrome c.
This article was published in Science
and referenced in Journal of AIDS & Clinical Research