alexa Markers of macrophage infiltration and measures of lipolysis in human abdominal adipose tissues.
Bioinformatics & Systems Biology

Bioinformatics & Systems Biology

Journal of Glycomics & Lipidomics

Author(s): Michaud A, Pelletier M, Nol S, Bouchard C, Tchernof A

Abstract Share this page

Abstract OBJECTIVE: We tested the hypothesis that high lipolytic responsiveness is related to increased expression of ATM genes in human adipose tissues. DESIGN AND METHODS: Omental (OM) and subcutaneous (SC) fat samples were obtained surgically in 46 women (age: 47.2 ± 4.7 years, BMI: 26.9 ± 5.2 kg/m(2) ). Body composition and fat distribution were measured using dual energy X-ray absorptiometry and computed tomography. Lipolysis was measured by glycerol release in mature adipocytes isolated by collagenase digestion under basal-, isoproterenol (10(-5) M)-, and forskolin (10(-5) M)-stimulated conditions. Quantification of macrophage gene mRNA expression (CD11b, CD11c, and CD68) in whole adipose tissue was performed using real-time RT-PCR. RESULTS: SC CD68 mRNA abundance was positively associated with isoproterenol-stimulated lipolysis (r = 0.36, P < 0.05). This association remained significant after adjustment for total body fat mass (r = 0.34, P ≤ 0.05). In the OM depot, CD11b mRNA abundance was positively associated with isoproterenol-stimulated lipolysis (r = 0.42, P ≤ 0.005). This association remained significant after adjustment for total body fat mass (r = 0.41, P ≤ 0.01). In subgroup analyses, high lipolytic rates in SC adipocytes were related to increased whole tissue expression of CD68 and CD11b in this compartment, independent of adiposity and fat cell size (P ≤ 0.001 and P ≤ 0.05). High lipolytic rates in OM adipocytes were related to increased whole tissue OM expression of CD11b, independent of adiposity and fat cell size (P ≤ 0.05). CONCLUSIONS: High adipocyte lipolytic responsiveness is related to increased expression of ATM markers in the corresponding compartment, independent of adiposity and fat cell size. Copyright © 2013 The Obesity Society. This article was published in Obesity (Silver Spring) and referenced in Journal of Glycomics & Lipidomics

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords