Author(s): Crutchfield CA, Lu W, Melamud E, Rabinowitz JD
Abstract Share this page
Abstract Driven by the advent of metabolomics, recent years have seen renewed interest in the investigation of yeast metabolism. Here we provide a practical guide to metabolomic analysis of yeast using liquid chromatography-mass spectrometry (LC-MS). We begin with background on LC-MS and its utility in studying yeast metabolism. We then describe key issues involved at each step of a typical yeast metabolomics experiment: in experimental design, cell culture, metabolite extraction, LC-MS, and data processing and analysis. Throughout, we highlight interdependencies between the steps that are relevant to developing an integrated workflow which effectively leverages LC-MS to reveal yeast biology. Copyright © 2010 Elsevier Inc. All rights reserved.
This article was published in Methods Enzymol
and referenced in Journal of Microbial & Biochemical Technology