alexa Mass spectrometry-based proteomic quest for diabetes biomarkers.
Chemical Engineering

Chemical Engineering

Mass Spectrometry & Purification Techniques

Author(s): Shao S, Guo T, Aebersold R

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Abstract Diabetes mellitus (DM) is a metabolic disorder characterized by chronic hyperglycemia, which affects hundreds of millions of individuals worldwide. Early diagnosis and complication prevention of DM are helpful for disease treatment. However, currently available DM diagnostic markers fail to achieve the goals. Identification of new diabetic biomarkers assisted by mass spectrometry (MS)-based proteomics may offer solution for the clinical challenges. Here, we review the current status of biomarker discovery in DM, and describe the pressure cycling technology (PCT)-Sequential Window Acquisition of all Theoretical fragment-ion (SWATH) workflow for sample-processing, biomarker discovery and validation, which may accelerate the current quest for DM biomarkers. This article is part of a Special Issue entitled: Medical Proteomics. Copyright © 2014 Elsevier B.V. All rights reserved. This article was published in Biochim Biophys Acta and referenced in Mass Spectrometry & Purification Techniques

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