alexa Measurement of ascorbic acid in human aqueous humour and plasma and bovine aqueous humour by high-performance liquid chromatography with electrochemical detection.
Pharmaceutical Sciences

Pharmaceutical Sciences

Pharmaceutica Analytica Acta

Author(s): Watson DG, Iqbal Z, Midgley JM, PryceJones H, Morrison L,

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Abstract High-performance liquid chromatography (HPLC) on ODS silica with cetyltrimethylammonium bromide as an ion-pairing reagent and electrochemical detection (ECD) was used to determine ascorbic acid in bovine and human aqueous humour and human plasma. Hydroquinone was used as the internal standard. A calibration curve plotted with ascorbic acid concentrations in the range 0.5-5 micrograms ml-1 for peak height versus internal standard peak height had a correlation coefficient of 0.998. The RSD (precision) between analyses of the same diluted sample was 1.5\% and the RSD (reproducibility) between analyses of separate aliquots of the same sample of aqueous humour was 1.6\%.
This article was published in J Pharm Biomed Anal and referenced in Pharmaceutica Analytica Acta

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