alexa Melatonin attenuates lung injury in a hind limb ischemia-reperfusion rat model.


Journal of Nephrology & Therapeutics

Author(s): Takhtfooladi H, Takhtfooladi M, Moayer F, Mobarakeh S

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Abstract OBJECTIVE: This study evaluated the protective antioxidant effect of melatonin on lung injury as a remote organ after skeletal muscle ischemia-reperfusion in rats. METHODS: Thirty male Wistar rats were allocated randomly into three experimental groups: operated with no ischemia (Sham) group, ischemia-reperfusion group and ischemia-reperfusion+melatonin group. Hind limb ischemia was induced by clamping the femoral artery. After 2h ischemia, the clamp was removed and the animal underwent 24h reperfusion. Rats in the ischemia-reperfusion + melatonin group received melatonin (10 mg/kg i.v.), immediately before the clamp was removed. At the end of the trial, animals were euthanized and the lungs were removed for water content determination, histopathological and biochemical studies. RESULTS: In the ischemia-reperfusion + melatonin group, tissues showed less intense histological abnormalities such as neutrophilic infiltration, intra-alveolar hemorrhage and edema compared with the ischemia-reperfusion group. Histopathologically, there was a significant difference (P < 0.05) between the two groups. The lung water content in the ischemia-reperfusion + melatonin group was significantly lower than the ischemia-reperfusion group (P < 0.05). Lung tissue myeloperoxidase (MPO) activity and nitric oxide (NO) level were significantly (P < 0.05) increased by ischemia-reperfusion. The increase in these parameters was reduced by melatonin. Comparing the ischemia-reperfusion+melatonin group with the sham group, no significant increase in all analyzed aspects of the research was observed. CONCLUSIONS: These findings suggest that melatonin has preventive effects in lung tissue injury after transient femoral artery occlusion. Copyright © 2013 Sociedade Portuguesa de Pneumologia. Published by Elsevier España, S.L.U. All rights reserved. This article was published in Rev Port Pneumol (2006) and referenced in Journal of Nephrology & Therapeutics

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