Author(s): Du ZF, Wales RG
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Abstract Samples of trophoblast recovered from the sheep conceptus on Day 13 of pregnancy formed spherical vesicles during culture in medium 199. These continued to expand and increase in dry weight over the next 6 days in vitro. After 6 days' culture, the metabolism of glucose by these vesicles was compared with that of Day-13 and Day-19 fresh trophoblastic tissue. The production of CO2 and lactate by vesicles was similar, although not identical, to production by Day-13 fresh tissue and did not exhibit the marked decrease in glucose catabolism seen in Day-19 trophoblast. The tissue from vesicles reacted to reduction in oxygen tension in a manner similar to the reaction of fresh tissue, with decreased glucose oxidation and increased glycolysis. The activity of the pentose phosphate pathway in vesicles was higher, and the activity in Day-19 fresh tissue was much lower, than that in Day-13 fresh trophoblast. Incorporation of glucose into the intracellular biochemical pools by vesicles was similar to incorporation into Day-13 fresh tissue. Limited observations were also made with vesicles derived from embryonic disc. Production of CO2 by these vesicles was intermediate between that detected in fresh Day-13 and Day-19 embryonic tissue. There were not significant differences in lactate production between fresh and cultured samples of embryonic tissue. These results show that vesicles formed in vitro remain metabolically active but do not mimic the biochemical changes seen in the tissue during development in vivo.
This article was published in Reprod Fertil Dev
and referenced in Single Cell Biology