alexa Microbial diversity in failed endodontic root-filled teeth.
Dentistry

Dentistry

JBR Journal of Interdisciplinary Medicine and Dental Science

Author(s): Zhang C, Hou BX, Zhao HY, Sun Z

Abstract Share this page

Abstract BACKGROUND: Persistent/secondary infections of human root canals play an important role in the failure of endodontic treatment. This study used 16S rRNA sequencing to assess microbial diversity in root-filled teeth associated with failed endodontic treatment. METHODS: DNA was extracted from 15 teeth with persistent intraradicular infections, and the 16S rRNA of all present bacteria were amplified by PCR, followed by cloning and sequencing of the 16S rRNA amplicons. RESULTS: All sample extracts were positive for PCR amplification using the universal 16S rRNA gene primers. Negative control reactions yielded no amplicons. Sixty-five phylotypes belonging to seven phyla were identified from 760 clones; a mean of 9.4 phylotypes were detected in each sample (range 3 - 15). Twenty-eight phylotypes were detected in more than one sample, revealing a high inter-sample variability. Parvimonas micra (60\%, 9/15), Solobacterium moore (47\%, 7/15), Dialister invisus (33\%, 5/15), Enterococcus faecalis (33\%, 5/15), Filifactor alocis (27\%, 4/15), and Fusobacterium nucleatum (27\%, 4/15) were the prevalent species. Nineteen as-yet-uncultivated phylotypes were identified, comprising a substantial proportion of the bacteria in many cases. CONCLUSIONS: Persistent intraradicular infections were present in all root-filled teeth associated with failed endodontic treatment. The current observations reveal new candidate endodontic pathogens, including as-yet-uncultivated bacteria and phylotypes that may participate in the mixed infections associated with post-treatment apical periodontitis.
This article was published in Chin Med J (Engl) and referenced in JBR Journal of Interdisciplinary Medicine and Dental Science

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords