alexa Microdissection techniques for molecular testing in surgical pathology.


Journal of Pulmonary & Respiratory Medicine

Author(s): Hunt JL, Finkelstein SD

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Abstract OBJECTIVE: To describe the techniques for microdissection of paraffin-embedded and frozen tissue sections for the use in molecular applications. DATA SOURCES: Original research papers and review papers and the authors' personal experiences. DATA SYNTHESIS: Manual and laser-capture microdissection are described in detail, with specific protocols for sample preparation and instructions for performing the microdissection. A section addressing frequently asked questions is also included. CONCLUSIONS: Microdissection is a technique that is very useful both in the research setting and for clinical molecular testing in paraffin-embedded tissue samples. The available techniques range from simple and inexpensive (manual microdissection) to complex and expensive (laser-capture microdissection). All of the techniques, however, require the user to be familiar with microscopy and histology. This article was published in Arch Pathol Lab Med and referenced in Journal of Pulmonary & Respiratory Medicine

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