alexa MicroRNA expression in the blood and brain of rats subjected to transient focal ischemia by middle cerebral artery occlusion.
Diabetes & Endocrinology

Diabetes & Endocrinology

Journal of Diabetes & Metabolism

Author(s): Jeyaseelan K, Lim KY, Armugam A

Abstract Share this page

Abstract BACKGROUND AND PURPOSE: Several hundred small RNAs called microRNAs (miRNAs) have been identified and characterized from various organisms, including humans. In humans, some of these miRNAs have been found to regulate (patho)physiologic conditions such as tumor progression/regression, cholesterol and glucose homeostasis, etc. In this report, we present data on the miRNAs expressed under ischemic conditions in both the brain and blood of rats subjected to middle cerebral artery occlusion (MCAo). METHODS: Sprague-Dawley rats subjected to MCAo were reperfused for either 24 or 48 hours, and both blood and brain samples were harvested. miRNA expression profiling and oligonucleotide microarray were carried out, and the data were validated by quantitative real-time polymerase chain reaction and correlated with published data on protein and gene expression in MCAo rats. RESULTS: We report here for the first time the involvement of miRNA regulation in brain pathogenesis associated with MCAo. Comparison with the corresponding DNA microarray data revealed that the target mRNA expression is correlated with the regulation of miRNA. We have also provided evidence that some of the miRNAs that are highly expressed in the ischemic brain can be detected in blood samples. CONCLUSIONS: Further studies are needed to evaluate the possible use of miRNAs as biomarkers in stroke and related pathologies. This article was published in Stroke and referenced in Journal of Diabetes & Metabolism

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords