alexa MinION Analysis and Reference Consortium: Phase 1 data release and analysis [version 1; referees: 2 approved]
Bioinformatics & Systems Biology

Bioinformatics & Systems Biology

Journal of Next Generation Sequencing & Applications

Author(s): Camilla LC Ip, Matthew Loose, ohn R Tyson, Mariateresa de Cesare, Bonnie L Brown, Miten Jain, Richard M Leggett, David A Eccles, Vadim Zalunin, John M Urban, Paolo Piazza, Rory J Bowden, Benedict Paten, Solomon Mwaigwisya, Elizabeth M Batty, Jared T Simpson, Terrance P Snutch, Ewan Birney, David Buck, Sara Goodwin, Hans J Jansen, Justin OGrady, Hugh E Olsen

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The advent of a miniaturized DNA sequencing device with a high-throughput contextual sequencing capability embodies the next generation of large scale sequencing tools. The MinION™ Access Programme (MAP) was initiated by Oxford Nanopore Technologies™ in April 2014, giving public access to their USB-attached miniature sequencing device. The MinION Analysis and Reference Consortium (MARC) was formed by a subset of MAP participants, with the aim of evaluating and providing standard protocols and reference data to the community. Envisaged as a multi-phased project, this study provides the global community with the Phase 1 data from MARC, where the reproducibility of the performance of the MinION was evaluated at multiple sites. Five laboratories on two continents generated data using a control strain of Escherichia coli K-12, preparing and sequencing samples according to a revised ONT protocol. Here, we provide the details of the protocol used, along with a preliminary analysis of the characteristics of typical runs including the consistency, rate, volume and quality of data produced. Further analysis of the Phase 1 data presented here, and additional experiments in Phase 2 of E. coli from MARC are already underway to identify ways to improve and enhance MinION performance.

This article was published in F1000 Research and referenced in Journal of Next Generation Sequencing & Applications

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