alexa Modulating effects of L-arginine on cytokine-stimulated lymphocyte migration in vitro.


Journal of Transplantation Technologies & Research

Author(s): Scott Isenberg J

Abstract Share this page

Abstract Elective microsurgical transplants have become routine. Yet there remains a 1-5\% rate of complete flap necrosis among these surgical reconstructions. This rate is much higher in emergent replantations. Despite technically accurate surgery, perfusion fails in this group. This lack of perfusion, or no-reflow, has been attributed to ischemic-reperfusion injury. The exact nature of this phenomenon remains poorly characterized, though it is clear that significant changes occur in such situations at the endothelial vascular interface. In an effort to understand the biomolecular events involved in ischemic-reperfusion injury we investigated the modulation of leukocyte transendothelial migration. Using a chemotactic chamber model with a cytokine stimulate mono-layer of umbilical vein endothelium, we evaluated the migration rate of peripheral blood mononuclear cells in the presence of exogenous L-arginine and/or the nitric oxide synthase inhibitor L-NAME. Levels of INF-gamma and TNF-alpha production were also determined. It was found that in the face of cytokine pre-stimulation and L-arginine, mononuclear cell trans-endothelial migration increased dramatically. There were also parallel increases in inflammatory cytokine output. These responses were sharply decreased by L-NAME. The results of this study suggest that in vitro nitric oxide augments transendothelial migration of inflammatory cells. Modulation of this response may provide a clinically useful method of minimizing ischemic-reperfusion injury. Copyright 2003 Wiley-Liss, Inc. This article was published in Microsurgery and referenced in Journal of Transplantation Technologies & Research

Relevant Expert PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version