alexa Molecular Analysis of IGH and Incomplete IGH D-J Clonality Gene Rearrangements in Hodgkin Lymphoma Malignancies.
Genetics

Genetics

Advancements in Genetic Engineering

Author(s): Ghorbian S, Jahanzad I, Estiar MA, Ziae JE, AsvadiKermani I,

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Abstract BACKGROUND: We evaluated molecular clonality in immunoglobulin heavy chain (IGH) and incomplete IGH D-J genes for improvement of clinical diagnosis of Hodgkin's lymphoma (HL). We applied BIOMED-2 protocols in HL cases, which were previously approved by clonality detection in non-Hodgkin lymphoma (NHL) cases. METHODS: We investigated 50 consecutive FFPE samples of classical HL (cHL) patients to assess IGH and IGH D-J clonal gene rearrangements by multiplex PCR protocols, which were provided by the European Biomedicine and Health (BIOMED-2) Concerted Action Project BMH4-CT98-3936. RESULTS: In the present study, there was a monoclonality of 86\% (43/50) including a clonality of 74\% (37/50) for IGH and a clonality of 42\% (21/50) in IGHD-J. In addition, a lack of clonality was detected in 14\% (7/50) of cases. Frequent gene rearrangements were detected in framework (FR) III (54\%) and FRII (20\%), whereas no clonality was seen in FRI. Furthermore, a monoclonality of 28\% and 14\% was detected in the DH(1-6)-JH and DH(see symbol)-JH gene rearrangements, respectively. CONCLUSIONS: The present study suggests that the complete IGH and incomplete IGH D-J clonality gene rearrangement assays using BIOMED-2 protocols could be considered a valuable method for detection of clonal gene rearrangements, especially in HL cases.
This article was published in Clin Lab and referenced in Advancements in Genetic Engineering

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