alexa Molecular characterization of Cucumber mosaic virus infecting Gladiolus, revealing its phylogeny distinct from the Indian isolate and alike the Fny strain of CMV.
Microbiology

Microbiology

Journal of Plant Pathology & Microbiology

Author(s): Dubey VK, Aminuddin, Singh VP

Abstract Share this page

Abstract The majority of Gladiolus plants growing in the botanical garden at NBRI, Lucknow, India and adjoining areas exhibited symptoms of mosaic, color breaking, stunting of spikes and reduction in flower size. The occurrence of Cucumber mosaic virus (CMV) was suspected in symptomatic Gladiolus plants. Cucumber mosaic virus, the type species of the genus Cucumovirus of the family Bromoviridae, is an important plant virus worldwide, which infects many plants and causes quantity and quality losses. For virus characterization, total RNA was isolated from leaves of infected plants and used in reverse transcriptase polymerase chain reaction with a primer set designed in the Cucumber mosaic virus coat protein region. Viral amplicons of the expected 657 bp size were obtained from infected plants. No viral amplicon was obtained from healthy control plants. Viral amplicons were cloned and sequenced (DQ295914). Molecular characterization was performed and phylogenetic relationship determined by the comparison of coat protein gene nucleotide and amino acid sequences with other Cucumber mosaic virus isolates reported from India and worldwide. The nucleotide and amino acid percentage comparison and phylogenetic tree results revealed that Cucumber mosaic virus infecting Gladiolus show resemblance with the Fny strain, which is not common in the Asian continent. This article was published in Virus Genes and referenced in Journal of Plant Pathology & Microbiology

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords