alexa Molecular cloning and complete nucleotide sequence of the attenuated rabies virus SAD B19
Veterinary Sciences

Veterinary Sciences

Research & Reviews: Journal of Veterinary Sciences


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Complementary DNA spanning the entire genome of the attenuated rabies virus strain SAD B 19 which is used for oral immunization of foxes in Europe was cloned and sequenced. The viral genome comprises 11,928 nucleotides and encodes the five viral proteins N, NS, M, G, and l. Deduced protein sequences are highly similar to those of the pathogenic PV strain, homologies ranging from 90.6% for the M to 98.6% for the l protein. The five cistrons are Separated by intergenic regions of 2, 5, 5, and 24 nucleotides, respectively. The G transcription stop/polyadenylation consensus signal in SAD B 19 is destroyed by a deletion of three A residues. The strong conservation of both noncoding and coding nucleotide sequences indicates a high selective pressure on the primary structure of rabies virus genomic RNA.

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This article was published in Virology and referenced in Research & Reviews: Journal of Veterinary Sciences

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