alexa mRNA expression of three glycosyltransferases in human hepatoma tissues.
Bioinformatics & Systems Biology

Bioinformatics & Systems Biology

Journal of Glycomics & Lipidomics

Author(s): Chen G, Guan M, Su B, Lu Y

Abstract Share this page

Abstract BACKGROUND: The sugar-chain structures of many glycoproteins are altered in the hepatoma tissues. The molecular mechanism by which these alterations occur remains largely unknown. METHODS: Messenger RNA expression of N-acetylglucosaminyltransferase (GlcNAcT-V), N-acetylglucosaminyltransferase III (GlcNAcT-III) and alpha1-6 fucosyltransferase (alpha1-6 FucT), were investigated in normal liver tissues samples (n=7), primary hepatic cancer (PHC) tissues (n=15) and noncancerous tissues surrounding PHC (n=15) by reverse transcriptase-polymerase chain reaction (RT-PCR). RESULTS: The mRNA expression of the three glycosyltransferases in PHC tissues were enhanced by 3- to 10-fold in comparison with that in normal liver tissues. There were significantly higher mRNA expressions of GlcNAcT-V in invasive PHC than non-invasive. CONCLUSIONS: The change of glycoprotein sugar-chain structures in PHC may be related to the abnormal mRNA expression of some glycosyltransferases and the levels of mRNA expression of GlcNAcT-V associated with PHC invasiveness.
This article was published in Clin Chim Acta and referenced in Journal of Glycomics & Lipidomics

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version