alexa Multiple, tandem plasmid integration in Saccharomyces cerevisiae.
Environmental Sciences

Environmental Sciences

Journal of Bioremediation & Biodegradation

Author(s): OrrWeaver TL, Szostak JW

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Abstract Nonreplicating plasmids transform Saccharomyces cerevisiae by recombining with a homologous site in the genome. Frequently, multiple copies of the plasmid integrate in a tandem array. We show that, after transformation with restriction enzyme-cut plasmids, most, if not all, multimers arise by sequential integration of plasmid molecules into the same genomic location.
This article was published in Mol Cell Biol and referenced in Journal of Bioremediation & Biodegradation

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